Procalcitonin
Kit Name : Joinstar Procalcitonin Detection Kit
Method: fluorescence dry quantitative immunoassay
Assay measuring range: 0.10ng/mL ~ 100.00ng/mL
Incubation time: 12min
Sample: human serum, plasma (EDTA anticoagulant、trisodium citrate), whole blood (EDTA anticoagulant、trisodium citrate)
Reference range: <0.50ng/mL
Storage and Stability:
✭Detection Buffer is stable for 12 months at 2°C ~8°C.
✭Sealed Test Device is stable for 12 months at 4°C～30°C.
• Procalcitonin (PCT) is the 116 amino acid precursor of the hormone calcitonin, produced by the C cells of the thyroid. Its synthesis is upregulated in bacterial infection.
• It is detectable within 2–4 h of infection, peaks within 6–24 h and can be present for up to 7 days.
• It has been proposed as a useful biomarker of bacterial infection and its use is related to reduced duration of antibiotic therapy.
• PCT is regarded as an early marker of sepsis in different settings, including ED, regular wards, surgical wards and ICU.
•《Surviving Sepsis Campaign: International Guidelines for the Management of Sepsis and Septic Shock（2021）》
♦ In a meta-analysis of 30 studies (3,244 patients), procalcitonin had a pooled sensitivity of 77% and specificity of 79% for sepsis in critically ill patients.
♦ The guidelines propose using procalcitonin and clinical based judgements to decide when to discontinue antimicrobials over clinical evaluation alone.
● Diagnosis of sepsis
As PCT levels are highly specific for sepsis caused by bacterial infection, PCT can be used as the biomarker to diagnose sepsis (at a cut-off of >2ng/mL) and differentiate severe bacterial infection.
● Assess the severity and progression of sepsis
It has been shown that increasing levels of PCT > (> 2 ng/mL) or are associated with increased disease severity Therefore PCT may be used to monitor disease progression.
● Sepsis prognosis
A rapid decline in PCT after treatment is usually associated with a favorable prognosis, whereas no reduction in PCT levels suggest a poor prognosis.
● PCT guided use of antibiotic therapy
♦ As an indicator of when to start antibiotic treatment.
♦ As a marker to judge the efficacy of antibiotics.
