Neutrophil Gelatinase-Associated Lipocalin
Kit Name: Joinstar Neutrophil Gelatinase-Associated Lipocalin Detection Kit
Method: fluorescence dry quantitative immunoassay
Assay measuring range: 50ng/mL ~ 5000ng/mL
Incubation time: 15min
Sample: Human serum ,plasma (EDTA anticoagulant), whole blood (EDTA anticoagulant),Urine
Reference range:
✭ Urine<100ng/mL
✭Human serum, plasma, whole blood<180ng/mL
Storage and Stability:
✭Detection Buffer is stable for 12 months at 2°C ~8°C.
✭Sealed Test Device is stable for 12 months at 4°C～30°C.
•Neutrophil gelatinase-associated lipocalin (NGAL) is a member of the lipid transport protein superfamily. It is a glycoprotein consisting of 178 amino acids and having a molecular weight of 25 kDa.
•It is expressed in activated neutrophils and certain epithelial cells, including renal tubular epithelial cells.
•Abnormal renal function causes damage to tubular epithelial cells, which secrete large amounts of NGAL. It is significantly elevated by different causes of kidney injury and is released into the urine and plasma.
•Normally, NGAL is maintained at a low level in urine and plasma, whereas it rises sharply within 2 hours after the onset of acute kidney injury (AKI). It is an excellent marker for the early diagnosis of AKI.
•Elevation of NGAL occurs earlier than serum creatinine (SCr) after kidney injury.
•《KDIGO Clinical Practice Guideline for Acute Kidney Injury（2012）》
The guideline recommended markers for kidney injury include NGAL, KIM-1 and lL-18.
Figure 1 .Conceptual model for AKI.
•《Expert Consensus on Clinical Diagnosis of Diabetic Kidney Disease in Chinese Adults (2016)》
The consensus recommends NGAL for the screening of diabetic kidney disease (DKD), especially for early screening of tubular involvement.
•Routine monitoring of critically ill patients
AKI has a prevalence of more than 50% in the ICU and a mortality rate of 50-80% in patients who are to be dialyzed. NGAL is capable of early diagnosis of AKI complications in critically ill patients.
•Early diagnosis of AKI after organ transplantation
Routine testing after kidney transplantation allows early assessment of the need for dialysis and functional recovery of the transplanted kidney within 1 week after surgery.
•Postoperative monitoring of coronary artery bypass grafting (CABG)
Earlier diagnosis of acute kidney injury in patients undergoing CABG can be achieved by measuring postoperative plasma NGAL concentration at 2 h.
•Early diagnosis of contrast-induced nephropathy (CIN)
✭Urinary NGAL of CIN patients showed significant changes 24h after angiography, while levels of other markers such as serum creatinine (SCr) concentration do not change significantly at this time.
✭NGAL rises rapidly in response to kidney injury, preceding changes in creatinine by as much as 2 to 3 days.
•Prediction of the risk of progression of chronic kidney disease (CKD)
The risk of CKD progression can increase by 3% and 2% for each 10 ng/mL increase in serum NGAL and urine NGAL concentrations, respectively.
