Kit Name: Joinstar D-Dimer Detection Kit
Method: fluorescence dry quantitative immunoassay
Assay measuring range: 50ng/mL ~ 10000ng/mL
Incubation time: 12min
Sample: Human plasma (Sodium citrate anticoagulant) and whole blood (Sodium citrate anticoagulant)
Reference range: <550ng/mL
Storage and Stability:
✭Detection Buffer is stable for 12 months at 2°C ~8°C.
✭Sealed Test Device is stable for 12 months at 4°C～30°C.
•The fibrin monomers crosslink with thrombin activated factor XIII to form cross-linked fibrin. D-Dimer is a final degradation product of the cross-linked fibrin that is hydrolyzed by fibrinolysin. It is one of the specific fibrinolytic process markers.
•The level of D-Dimer in the peripheral blood of healthy people is very low, and it increases significantly in various hypercoagulable states and secondary hyperfibrinolysis
•D-Dimer has a half-life of approximately 8 hours in the circulating blood and is cleared by the kidneys and reticuloendothelial system.
•《Guidelines for the diagnosis and management of disseminated intravascular coagulation (DIC)（2009）》
Tests for FDP or D-dimers may also be helpful to differentiate DIC from other conditions that are associated with a low platelet count or prolonged clotting times, such as chronic liver disease.
•《Quantitative D-Dimer for the Exclusion of Venous Thromboembolic Disease （GLSI H59-P）》
The quantitative detection of D-Dimer at low and medium peak clinical risk can be used as an exclusionary diagnosis for venous thromboembolism (VTE).
•《2014 ESC guidelines on the diagnosis and management of acute pulmonary embolism》
D-Dimer test is recommended as a screening indicator for the diagnosis of pulmonary embolism (PE) in the emergency department.
•《The Chinese expert consensus on the diagnosis and treatment of acute hemorrhagic coagulopathy（2020）》
D-dimer is a more reliable indicator of thrombotic risk, and plasma D-dimer levels < 500 ng/mL are often used clinically as the cut-off value to rule out thrombosis.
•Exclusion diagnosis of venous thromboembolism (VTE)
The most important clinical value of D-dimer testing is for the exclusion of venous thrombotic disease (VTE).
•Prediction of Venous Thromboembolism (VTE)
In patients hospitalized with acute illness, elevated D-Dimer levels at admission (>500 ng/mL) can be used as an indicator for the subsequent development of VTE.
•Diagnosis of diffuse intravascular coagulation (DIC)
D-Dimer is elevated at the early stage of DIC and can continue to rise as the disease progresses, it is therefore an important indicator for the diagnosis, severity and prognosis of DIC.
Screening and exclusion of acute aortic dissection (AAD), adjuvant diagnosis of acute coronary syndrome (ACS), diagnosis and prognostic judgment of cerebral infarction, adjuvant diagnosis and prognostic evaluation of sepsis, monitoring of hyperemesis, adjuvant diagnosis of malignancy, post-surgical thrombosis monitoring, monitoring of thrombolytic therapy, etc.
Figure 1. D-Dimer Formation